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Cremaster muscle perfusion, oxygenation, and heterogeneity revealed by a new automated acquisition system in a rodent model of prolonged hemorrhagic shock.
Torres Filho, Ivo P; Barraza, David; Hildreth, Kim; Williams, Charnae; Dubick, Michael A.
Afiliación
  • Torres Filho IP; Damage Control Resuscitation, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas.
  • Barraza D; Damage Control Resuscitation, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas.
  • Hildreth K; Damage Control Resuscitation, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas.
  • Williams C; Damage Control Resuscitation, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas.
  • Dubick MA; Damage Control Resuscitation, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas.
J Appl Physiol (1985) ; 127(6): 1548-1561, 2019 12 01.
Article en En | MEDLINE | ID: mdl-31670599
ABSTRACT
Local blood flow/oxygen partial pressure (Po2) distributions and flow-Po2 relationships are physiologically relevant. They affect the pathophysiology and treatment of conditions like hemorrhagic shock (HS), but direct noninvasive measures of flow, Po2, and their heterogeneity during prolonged HS are infrequently presented. To fill this void, we report the first quantitative evaluation of flow-Po2 relationships and heterogeneities in normovolemia and during several hours of HS using noninvasive, unbiased, automated acquisition. Anesthetized rats were subjected to tracheostomy, arterial/venous catheterizations, cremaster muscle exteriorization, hemorrhage (40% total blood volume), and laparotomy. Control animals equally instrumented were not subjected to hemorrhage/laparotomy. Every 0.5 h for 4.5 h, noninvasive laser speckle contrast imaging and phosphorescence quenching were employed for nearly 7,000 flow/Po2 measurements in muscles from eight animals, using an automated system. Precise alignment of 16 muscle areas allowed overlapping between flow and oxygenation measurements to evaluate spatial heterogeneity, and repeated measurements were used to estimate temporal heterogeneity. Systemic physiological parameters and blood chemistry were simultaneously assessed by blood samplings replaced with crystalloids. Hemodilution was associated with local hypoxia, but increased flow prevented major oxygen delivery decline. Adding laparotomy and prolonged HS resulted in hypoxia, ischemia, decreased tissue oxygen delivery, and logarithmic flow/Po2 relationships in most regions. Flow and Po2 spatial heterogeneities were higher than their respective temporal heterogeneities, although this did not change significantly over the studied period. This quantitative framework establishes a basis for evaluating therapies aimed at restoring muscle homeostasis, positively impacting outcomes of civilian and military trauma/HS victims.NEW & NOTEWORTHY This is the first study on flow-Po2 relationships during normovolemia, hemodilution, and prolonged hemorrhagic shock using noninvasive methods in multiple skeletal muscle areas of monitored animals. Automated flow/Po2 measurements revealed temporal/spatial heterogeneities, hypoxia, ischemia, and decreased tissue oxygen delivery after trauma/severe hemorrhage. Hemodilution was associated with local hypoxia, but hyperemia prevented a major decline in oxygen delivery. This framework provides a quantitative basis for testing therapeutics that positively impacts muscle homeostasis and outcomes of trauma/hemorrhagic shock victims.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Roedores / Choque Hemorrágico / Músculos Abdominales Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Roedores / Choque Hemorrágico / Músculos Abdominales Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2019 Tipo del documento: Article