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Engineering geometrical 3-dimensional untethered in vitro neural tissue mimic.
Pagan-Diaz, Gelson J; Ramos-Cruz, Karla P; Sam, Richard; Kandel, Mikhail E; Aydin, Onur; Saif, M Taher A; Popescu, Gabriel; Bashir, Rashid.
Afiliación
  • Pagan-Diaz GJ; Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Ramos-Cruz KP; Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Sam R; School of Molecular and Cellular Biology, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Kandel ME; Department of Electrical and Computer Engineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Aydin O; Department of Mechanical Science and Engineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Saif MTA; Department of Mechanical Science and Engineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Popescu G; Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
  • Bashir R; Department of Electrical and Computer Engineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801.
Proc Natl Acad Sci U S A ; 116(51): 25932-25940, 2019 12 17.
Article en En | MEDLINE | ID: mdl-31796592
Formation of tissue models in 3 dimensions is more effective in recapitulating structure and function compared to their 2-dimensional (2D) counterparts. Formation of 3D engineered tissue to control shape and size can have important implications in biomedical research and in engineering applications such as biological soft robotics. While neural spheroids routinely are created during differentiation processes, further geometric control of in vitro neural models has not been demonstrated. Here, we present an approach to form functional in vitro neural tissue mimic (NTM) of different shapes using stem cells, a fibrin matrix, and 3D printed molds. We used murine-derived embryonic stem cells for optimizing cell-seeding protocols, characterization of the resulting internal structure of the construct, and remodeling of the extracellular matrix, as well as validation of electrophysiological activity. Then, we used these findings to biofabricate these constructs using neurons derived from human embryonic stem cells. This method can provide a large degree of design flexibility for development of in vitro functional neural tissue models of varying forms for therapeutic biomedical research, drug discovery, and disease modeling, and engineering applications.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Técnicas de Cultivo de Tejidos / Andamios del Tejido / Tejido Nervioso Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Técnicas de Cultivo de Tejidos / Andamios del Tejido / Tejido Nervioso Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2019 Tipo del documento: Article