IL-10 induces TGF-ß secretion, TGF-ß receptor II upregulation, and IgA secretion in B cells.
Eur Cytokine Netw
; 30(3): 107-113, 2019 09 01.
Article
en En
| MEDLINE
| ID: mdl-31957700
ABSTRACT
Interleukin-10 (IL-10) is a pleiotropic cytokine, which has both regulatory and stimulatory effects on different immune cell types. Different studies have reported the importance of IL-10 and Transforming growth factor-beta (TGF-ß) in the regulation of B cell class switching the production of immunoglobulin A (IgA); however, the underlying mechanisms remain to be fully elucidated. The objective of this study was to investigate the TGF-ß response during B stimulation of human B cells by IL-10. Pan B cells of healthy donors were negatively purified by a magnetic cell separation technique. B cells were cultured with multimeric CD40 ligand (mCD40L) and IL-10 for two and seven days. After harvesting in specific days, TGF-ß receptor II and surface IgA expression was determined by flow cytometry, while IgA and TGF-ß secretion was assessed by enzyme-linked immunosorbent assay. B cells endogenously expressed TGF-ß receptor II and after 48 hours cultivation with mCD40L or mCD40L plus IL-10, both the expression of this receptor and the production of TGF-ß were significantly increased. Notably, TGF-ß levels following stimulation with mCD40L and IL-10 were higher than those produced by B cells stimulated with mCD40L alone. Furthermore, at day 7 and following IL-10 stimulation, there was a significant rise in the amount of IgA secretion by class-switched plasma cells, which was higher than stimulation with mCD40L alone. Our findings suggest that IL-10 can modulate TGF-ß production and TGF-ß receptor expression in mCD40-activated human B lymphocytes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Inmunoglobulina A
/
Linfocitos B
/
Regulación hacia Arriba
/
Factor de Crecimiento Transformador beta
/
Interleucina-10
/
Receptores de Factores de Crecimiento Transformadores beta
Límite:
Humans
Idioma:
En
Revista:
Eur Cytokine Netw
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Irán