A new analysis method for the membrane viscosity from steady-state fluorescence depolarization.
Biorheology
; 25(1-2): 253-9, 1988.
Article
en En
| MEDLINE
| ID: mdl-3196822
ABSTRACT
The absolute value of the viscosity in membrane lipid bilayers, which is different from the microviscosity advocated by Shinitzky, could be calculated from steady-state fluorescence depolarization of a hydrocarbon fluorophore, 1,6-diphenyl-1,3,5-hexatriene (DPH). This method was based on the theory of time-resolved fluorescence anisotropy and empirical relationships between fluorescence life time and the anisotropy parameters such as half cone angle in wobbling motion and wobbling diffusion rate of the fluorescent probe. Obtained viscosity values of various membranes from this method were consistent with those from time resolved method within experimental error.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polarización de Fluorescencia
/
Fluidez de la Membrana
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biorheology
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón