[The effect of peroxiredoxin 2 on transforming growth factor-ß1-induced fibroblast proliferation and collagen synthesis].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 38(1): 7-12, 2020 Jan 20.
Article
en Zh
| MEDLINE
| ID: mdl-32062888
ABSTRACT
Objective:
To investigate the effect of peroxiredoxin 2 (Prx2) overexpression on fibroblast proliferation and collagen synthesis induced by transforming growth factor-ß1 (TGF-ß1) .Methods:
Fibroblasts were randomly divided into control group (DMEM medium) , TGF-ß1 group (5 µg/L TGF-ß1) , negative control group (treated with 5 µg/L TGF-ß1 and transfected with empty lentiviral vector) , and Prx2 group (treated with 5 µg/L TGF-ß1 and transfected with Prx2 overexpression lentiviral vector) . MTT assay was used to measure cell proliferation, immunofluorescence assay was used to measure the expression of 8-OHdG, and Western blot was used to measure the expression of p-JNK, p-P38, collagen type I, collagen type III, and Prx2. SPSS 18.0 was used for statistical analysis. The continuous data were expressed as mean±standard deviation; an analysis of variance was used for comparison between groups, and the least significant difference t-test was used for further comparison between two groups.Results:
Lentiviral transfection was performed successfully, and the Prx2 group had a significant increase in the protein expression of Prx2 (P<0.05) . Compared with the control group, the TGF-ß1 group had a significant increase in the proliferation ability (P<0.05) , and compared with the TGF-ß1 group, the Prx2 group had a significant reduction in the proliferation ability (P<0.05) . Compared with the control group, the TGF-ß1 group had significant increases in the expression of 8-OHdG, p-JNK, p-P38, collagen type I, and collagen type III (P<0.05) ; compared with the TGF-ß1 group, the negative control group had no significant changes in the expression of 8-OHdG, p-JNK, p-P38, collagen type I, and collagen type III (P>0.05) , while the Prx2 group had significant reductions in the above parameters (P<0.05) .Conclusion:
Prx2 overexpression inhibits fibroblast proliferation and collagen synthesis induced by TGF-ß1 through inhibiting reactive oxygen species and activating the JNK and P38 pathways.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Colágeno Tipo I
/
Proliferación Celular
/
Factor de Crecimiento Transformador beta1
/
Peroxirredoxinas
/
Fibroblastos
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Asunto de la revista:
MEDICINA OCUPACIONAL
Año:
2020
Tipo del documento:
Article
País de afiliación:
China