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Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes.
Kafader, Jared O; Melani, Rafael D; Durbin, Kenneth R; Ikwuagwu, Bon; Early, Bryan P; Fellers, Ryan T; Beu, Steven C; Zabrouskov, Vlad; Makarov, Alexander A; Maze, Joshua T; Shinholt, Deven L; Yip, Ping F; Tullman-Ercek, Danielle; Senko, Michael W; Compton, Philip D; Kelleher, Neil L.
Afiliación
  • Kafader JO; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA.
  • Melani RD; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA.
  • Durbin KR; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA.
  • Ikwuagwu B; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
  • Early BP; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA.
  • Fellers RT; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA.
  • Beu SC; S.C. Beu Consulting, Austin, TX, USA.
  • Zabrouskov V; Thermo Fisher Scientific, San Jose, CA, USA.
  • Makarov AA; Thermo Fisher Scientific, Bremen, Germany.
  • Maze JT; Thermo Fisher Scientific, Austin, TX, USA.
  • Shinholt DL; Thermo Fisher Scientific, Austin, TX, USA.
  • Yip PF; Thermo Fisher Scientific, San Jose, CA, USA.
  • Tullman-Ercek D; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
  • Senko MW; Thermo Fisher Scientific, San Jose, CA, USA.
  • Compton PD; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA. philip-compton@northwestern.edu.
  • Kelleher NL; Departments of Chemistry, Molecular Biosciences, The Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.
Nat Methods ; 17(4): 391-394, 2020 04.
Article en En | MEDLINE | ID: mdl-32123391
An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I2MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría de Masas / Proteínas / Proteómica Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría de Masas / Proteínas / Proteómica Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos