AAAAI, American Academy of Allergy Asthma and Immunology; ABA, Allergen Bead Array; ACAAI, American College of Allergy Asthma and Immunology; AEC, Allergen Exposure Chambers; AIT, allergen immunotherapy; AP, Alkaline Phosphatase; AU/mL, Allergenic Units milliLiter; Allergy; Anti-IgE, Antibody against IgE; BAT, Basophil Activation Test; BAU/mL, Biologic Allergenic Units milliLiter; CBA, Cytometric Bead Array; CCD, Cross-reactive Carbohydrate Determinants; CDER, Center for Drug Evaluation and Research (USA); CL, Chemiluminescence; CaFE, Calibrated Fluorescence Enhancement; DBPCFC, Double-Blind Placebo-Controlled Food Challenge; Diagnostic strategies; EAACI, European Academy of Allergy and Immunology; EIA, Enzyme Immune Assay; ELISA, Enzyme Linked Immuno Sorbent Analysis; EMEA, European MEdicine Agencies; ENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3; FACS, Fluorescence-Activated Cell Sorting; FDA, Food and Drug Administration (U.S. Department of Health and Human Services); FEIA, Fluorescent Enzyme Immunoassays; FcεRI, High affinity IgE receptor; H1, Histamine 1 receptor; H2, Histamine 2 receptor; HPO, Horseradish Peroxidase; IDT, Intradermal Test; ISAC, Immuno-Solid phase Allergen Chip; IUIS, International Union of Immunological Societies; IVD, in vitro diagnostic tool; IgE; IgE, immunoglobulin E; In vitro tests; LAMP-3, Lysosomal-Associated Membrane Protein; MBAD, Molecule Based Allergy Diagnostics; MRGPRX2, Mas-related G protein receptor 2; NIH, National Institutes of Health (USA); NMBAs, NeuroMuscular Blocking Agents; NPA, Negative Percent Agreement; NSAIDs, Non-Steroidal Anti-Inflammatory Drugs; PPA, Positive Percent Agreement; PPT, Prick-Prick Test; RAST, Radio Allergo Sorbent Test; SCAR, severe cutaneous adverse drug reactions; SPT, Skin prick test; Skin tests; kUA/L, kilo Units of Allergen/Liter for allergen-specific IgE antibody assays; mAb, Monoclonal Antibody; pNPP, p-Nitrophenylphosphate; sIgE, specific IgE; w/v, weight /volume