[Effect of knockdown of O-Glcnac transferase on hepatocyte fat synthesis].
Zhonghua Gan Zang Bing Za Zhi
; 28(2): 147-151, 2020 Feb 20.
Article
en Zh
| MEDLINE
| ID: mdl-32164066
ABSTRACT
Objective:
To investigate the effect of knockdown of O-GlcNAc transferase (OGT) on hepatocyte fat synthesis.Methods:
Liver cell line L02 were used to established the model of hepatic steatosis. The levels of OGT and O-GlcNAc protein were detected by Western blot. The OGT knockdown cell line of L02 cells was established, and its lipid formation ability was detected after induction of oleic acid (OA). Real-time quantitative PCR (qRT-PCR) and Western blot were used to detect mRNA and protein expression of enzymes related to fat synthesis. An independent sample t test was used.Results:
Western blot showed that the expression of OGT and O-GlcNAc was increased in L02 cells after adipogenesis (P < 0.05). After shOGT lentivirus infects L02 cells, OGT mRNA levels were down-regulated (P < 0.01). Oil red O staining showed that the lipid in L02 shOGT cells decreased, qRT-PCR showed that the mRNA expressions of fat synthase (ACC1), (FASN) and (SCD1) were decreased, the difference was statistically significant (P < 0.05), protein Expression is consistent with mRNA expression.Conclusion:
Knockdown of OGT can inhibit hepatocyte fat synthesis by reducing O-GlcNAc levels.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
N-Acetilglucosaminiltransferasas
/
Hepatocitos
/
Histona Acetiltransferasas
/
Hígado Graso
/
Hialuronoglucosaminidasa
/
Antígenos de Neoplasias
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2020
Tipo del documento:
Article