Multi-Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing.

Ali, Amr S; Chen, Rachel; Raju, Ravali; Kshirsagar, Rashmi; Gilbert, Alan; Zang, Li; Karger, Barry L; Ivanov, Alexander R

Ali, Amr S; Chen, Rachel; Raju, Ravali; Kshirsagar, Rashmi; Gilbert, Alan; Zang, Li; Karger, Barry L; Ivanov, Alexander R.

Afiliación

Ali AS; Cell Culture Development, Biogen Inc., Cambridge, MA, 02142, USA.
Chen R; Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA.
Raju R; Analytical Development, Biogen Inc., Cambridge, MA, 02142, USA.
Kshirsagar R; Analytical Development, Biogen Inc., Cambridge, MA, 02142, USA.
Gilbert A; Cell Culture Development, Biogen Inc., Cambridge, MA, 02142, USA.
Zang L; Cell Culture Development, Biogen Inc., Cambridge, MA, 02142, USA.
Karger BL; Cell Culture Development, Biogen Inc., Cambridge, MA, 02142, USA.
Ivanov AR; Analytical Development, Biogen Inc., Cambridge, MA, 02142, USA.

Biotechnol J ; 15(8): e1900565, 2020 Aug.

Article en En | MEDLINE | ID: mdl-32170810

Asunto(s)

Biología Computacional; Cisteína; Metabolismo Energético; Animales; Células CHO; Biología Computacional/métodos; Cricetinae; Cricetulus; Cisteína/química; Oxidación-Reducción

Palabras clave

Chinese hamster ovary bioprocessing; endoplasmic reticulum stress; energy metabolism; monoclonal antibodies; multi-omics; redox

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Biología Computacional / Cisteína / Metabolismo Energético Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biotechnol J Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google