Effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on excisional wound healing in a rat's model.

ABSTRACT

OBJEVTIVE To investigate the effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on the excisional wound healing in a rat's model. METHODS: Rats were performed a surgical lesion with a 2.0 cm resected tissue in the dorsal fascia. Following, animals were randomly divided into model group, YNB group and APE group those were respectively treated with saline, 1 mg/mL of Yunnan Baiyao and 1 mg/mL of Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) extracts. Wound contractions in days 0, 3, 7, 14 and 21 were calculated by an image analyzer. Histological analysis was analyzed using hematoxilin and eosin. Levels of tumor necrosis factor α (TNF-α), interleukin-2 (IL-2), IL-4, IL-10, transforming growth factor-ß1 (TGF-ß1) and basic fibroblast growth factor (bFGF) were determined by real-time quantitative PCR. RESULTS: Compared with that of YNB group and APE gtoup, the skin of rats showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast in the model group. In the APE group, levels of TNF-α and IL-2 were significantly down-regulated and IL-4 and IL-10 significantly up-regulated in contrasted with that of model group. In addition, levels of TGF-ß1 and bFGF in the APE group were significantly induced compared with that of model group. CONCLUSION: The results suggest that the extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) promote wound healing in the rats, which is associated with enhancing anti-inflammatory ability and inducing fibroblast formation.