Your browser doesn't support javascript.
loading
Intravital imaging of mouse embryos.
Huang, Qiang; Cohen, Malkiel A; Alsina, Fernando C; Devlin, Garth; Garrett, Aliesha; McKey, Jennifer; Havlik, Patrick; Rakhilin, Nikolai; Wang, Ergang; Xiang, Kun; Mathews, Parker; Wang, Lihua; Bock, Cheryl; Ruthig, Victor; Wang, Yi; Negrete, Marcos; Wong, Chi Wut; Murthy, Preetish K L; Zhang, Shupei; Daniel, Andrea R; Kirsch, David G; Kang, Yubin; Capel, Blanche; Asokan, Aravind; Silver, Debra L; Jaenisch, Rudolf; Shen, Xiling.
Afiliación
  • Huang Q; Department of Pediatric Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. drhuang@xjtu.edu.cn jaenisch@wi.mit.edu xs37@duke.edu.
  • Cohen MA; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Alsina FC; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Devlin G; Department of Molecular Genetics and Microbiology, School of Medicine, Duke University, Durham, NC, USA.
  • Garrett A; Department of Surgery, School of Medicine, Duke University, Durham, NC, USA.
  • McKey J; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Havlik P; Department of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.
  • Rakhilin N; Department of Surgery, School of Medicine, Duke University, Durham, NC, USA.
  • Wang E; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Xiang K; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Mathews P; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Wang L; Division of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, Durham, NC, USA.
  • Bock C; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Ruthig V; Duke Cancer Institute, School of Medicine, Duke University, Durham, NC, USA.
  • Wang Y; Department of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.
  • Negrete M; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Wong CW; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Murthy PKL; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Zhang S; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
  • Daniel AR; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Kirsch DG; Department of Radiation Oncology, Duke University Medical Center, Durham, NC, USA.
  • Kang Y; Department of Radiation Oncology, Duke University Medical Center, Durham, NC, USA.
  • Capel B; Department of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, NC, USA.
  • Asokan A; Division of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, Durham, NC, USA.
  • Silver DL; Department of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.
  • Jaenisch R; Department of Surgery, School of Medicine, Duke University, Durham, NC, USA.
  • Shen X; Department of Molecular Genetics and Microbiology, School of Medicine, Duke University, Durham, NC, USA.
Science ; 368(6487): 181-186, 2020 04 10.
Article en En | MEDLINE | ID: mdl-32273467
Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Desarrollo Embrionario / Embrión de Mamíferos / Microscopía Intravital / Cresta Neural Límite: Animals / Pregnancy Idioma: En Revista: Science Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Desarrollo Embrionario / Embrión de Mamíferos / Microscopía Intravital / Cresta Neural Límite: Animals / Pregnancy Idioma: En Revista: Science Año: 2020 Tipo del documento: Article