Proteomics analysis of potential serum biomarkers for insulin resistance in patients with polycystic ovary syndrome.
Int J Mol Med
; 45(5): 1409-1416, 2020 May.
Article
en En
| MEDLINE
| ID: mdl-32323743
ABSTRACT
The aim of the present study was to identify potential serum biomarkers for insulin resistance (IR) in patients with polycystic ovary syndrome (PCOS) by comparing the differences in serum protein expression levels between PCOS patients with and without IR. PCOS patients aged from 18 to 35 years were recruited at Guangdong Women and Children's Hospital from January, 2013 to February, 2014. A total of 218 PCOS patients were enrolled and divided into the insulin resistance (PCOSIR) and noninsulin resistance (PCOSNIR) groups according to their homeostasis model assessment of insulin resistance. Twodimensional difference gel electrophoresis (2DDIGE) and matrixassisted laser desorption/ionization timeofflight mass spectrometry (MALDITOFMS/MS) techniques were used to identify differences in protein expression levels between the PCOSIR and PCOSNIR groups. The present study demonstrated that the total cholesterol (TCH), triglycerides (TG), lowdensity lipoprotein (LDL), fasting plasma glucose (FPG), 3h blood glucose (3hBG) and uric acid (UA) levels in the PCOSIR group were higher than those in the PCOSNIR group (P<0.05). Between the PCOSIR and PCOSNIR groups, a total of 20 differentially expressed protein spots were detected by 2DDIGE. Among these, 4 proteins, namely afamin, serotransferrin, complement C3 and apolipoprotein C3 (APOC3), were also identified by MALDITOFMS/MS. The alteration of APOC3 was further confirmed by western blot analysis and enzymelinked immunosorbent assay (ELISA). The present study also confirmed that the expression level of APOC3 was positively associated with the homeostasis model assessment of insulin resistance (HOMAIR). On the whole, the data indicate that APOC3 may be a potential diagnostic marker for PCOSIR patients.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Síndrome del Ovario Poliquístico
/
Resistencia a la Insulina
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Biomarcadores
Límite:
Adult
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Female
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Humans
Idioma:
En
Revista:
Int J Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
2020
Tipo del documento:
Article