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Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2.
Rampello, Anthony J; Laudermilch, Ethan; Vishnoi, Nidhi; Prophet, Sarah M; Shao, Lin; Zhao, Chenguang; Lusk, C Patrick; Schlieker, Christian.
Afiliación
  • Rampello AJ; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.
  • Laudermilch E; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.
  • Vishnoi N; Department of Cell Biology, Yale School of Medicine, New Haven, CT.
  • Prophet SM; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.
  • Shao L; Department of Neuroscience, Yale School of Medicine, New Haven, CT.
  • Zhao C; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.
  • Lusk CP; Department of Cell Biology, Yale School of Medicine, New Haven, CT.
  • Schlieker C; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.
J Cell Biol ; 219(6)2020 06 01.
Article en En | MEDLINE | ID: mdl-32342107
ABSTRACT
Nuclear envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the phenotypic hallmark of Torsin ATPase manipulation. Both the dynamics of blebbing and the connection to nuclear pore biogenesis remain poorly understood. We employ a proteomics-based approach to identify myeloid leukemia factor 2 (MLF2) as a luminal component of the bleb. Using an MLF2-based live-cell imaging platform, we demonstrate that nuclear envelope blebbing occurs rapidly and synchronously immediately after nuclear envelope reformation during mitosis. Bleb formation is independent of ubiquitin conjugation within the bleb, but strictly dependent on POM121, a transmembrane nucleoporin essential for interphase nuclear pore biogenesis. Nup358, a late marker for interphase nuclear pore complex (NPC) biogenesis, is underrepresented relative to FG-nucleoporins in nuclear envelopes of Torsin-deficient cells. The kinetics of bleb formation, its dependence on POM121, and a reduction of mature NPCs in Torsin-deficient cells lead us to conclude that the hallmark phenotype of Torsin manipulation represents aberrant NPC intermediates.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Nucleares / Chaperonas Moleculares / Poro Nuclear / Membrana Nuclear Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Cell Biol Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Nucleares / Chaperonas Moleculares / Poro Nuclear / Membrana Nuclear Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Cell Biol Año: 2020 Tipo del documento: Article