SON DNA-binding protein mediates macrophage autophagy and responses to intracellular infection.
FEBS Lett
; 594(17): 2782-2799, 2020 09.
Article
en En
| MEDLINE
| ID: mdl-32484234
Intracellular pathogens affect diverse host cellular defence and metabolic pathways. Here, we used infection with Francisella tularensis to identify SON DNA-binding protein as a central determinant of macrophage activities. RNAi knockdown of SON increases survival of human macrophages following F. tularensis infection or inflammasome stimulation. SON is required for macrophage autophagy, interferon response factor 3 expression, type I interferon response and inflammasome-associated readouts. SON knockdown has gene- and stimulus-specific effects on inflammatory gene expression. SON is required for accurate splicing and expression of GBF1, a key mediator of cis-Golgi structure and function. Chemical GBF1 inhibition has similar effects to SON knockdown, suggesting that SON controls macrophage functions at least in part by controlling Golgi-associated processes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Autofagia
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Antígenos de Histocompatibilidad Menor
/
Factores de Intercambio de Guanina Nucleótido
/
Proteínas de Unión al ADN
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Interacciones Huésped-Patógeno
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Francisella tularensis
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Aparato de Golgi
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Macrófagos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
FEBS Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos