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SON DNA-binding protein mediates macrophage autophagy and responses to intracellular infection.
Gregory, David J; DeLoid, Glen M; Salmon, Sharon L; Metzger, Dennis W; Kramnik, Igor; Kobzik, Lester.
Afiliación
  • Gregory DJ; Molecular and Physiological Sciences Program, Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
  • DeLoid GM; Pediatric Infectious Disease, Massachusetts General Hospital, Boston, MA, USA.
  • Salmon SL; Molecular and Physiological Sciences Program, Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
  • Metzger DW; Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY, USA.
  • Kramnik I; Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY, USA.
  • Kobzik L; Pulmonary Center, Department of Medicine, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, MA, USA.
FEBS Lett ; 594(17): 2782-2799, 2020 09.
Article en En | MEDLINE | ID: mdl-32484234
Intracellular pathogens affect diverse host cellular defence and metabolic pathways. Here, we used infection with Francisella tularensis to identify SON DNA-binding protein as a central determinant of macrophage activities. RNAi knockdown of SON increases survival of human macrophages following F. tularensis infection or inflammasome stimulation. SON is required for macrophage autophagy, interferon response factor 3 expression, type I interferon response and inflammasome-associated readouts. SON knockdown has gene- and stimulus-specific effects on inflammatory gene expression. SON is required for accurate splicing and expression of GBF1, a key mediator of cis-Golgi structure and function. Chemical GBF1 inhibition has similar effects to SON knockdown, suggesting that SON controls macrophage functions at least in part by controlling Golgi-associated processes.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Antígenos de Histocompatibilidad Menor / Factores de Intercambio de Guanina Nucleótido / Proteínas de Unión al ADN / Interacciones Huésped-Patógeno / Francisella tularensis / Aparato de Golgi / Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: FEBS Lett Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Antígenos de Histocompatibilidad Menor / Factores de Intercambio de Guanina Nucleótido / Proteínas de Unión al ADN / Interacciones Huésped-Patógeno / Francisella tularensis / Aparato de Golgi / Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: FEBS Lett Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos