Pukyongiella litopenaei gen. nov., sp. nov., a novel bacterium isolated from the gut content of a whiteleg shrimp Litopenaeus vannamei.
Int J Syst Evol Microbiol
; 70(7): 4193-4198, 2020 Jul.
Article
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| MEDLINE
| ID: mdl-32539910
ABSTRACT
A Gram-negative, aerobic bacterium, designated as SH-1T, was isolated from the gut content of a whiteleg shrimp, Litopenaeus vannamei collected in a shrimp farm in South Korea. The bacterial cells were ovoid rod-shaped, non-motile, oxidase-positive and catalase-negative. Growth was observed at 20-35 °C (optimum, 30 °C), pH 5.0-9.5 (pH 8.5) and in the presence of 0-6â% (w/v) NaCl (2-3â%). The major polar lipids were phosphatidylglycerol, phosphatidylinositolmannoside, unidentified aminolipid and two unidentified lipids. The G+C content was 66.1 mol% and the predominant respiratory quinone was Q-10. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SH-1T was placed in a distinct clade with Primorskyibacter marinus PX7T (96.97â% sequence similarity), Pontibaca methylaminivorans DSM 21219T (96.03â%) and Pelagivirga sediminicola BH-SD19T (95.02â%) in the family Rhodobacteraceae and distantly related with them to be a new genus. The digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) values calculated from whole-genome-sequence comparison between the SH-1T and the close species were in the ranges of 19.0-19.8, 73.8-74.9 and 64.1-65.9â%, respectively. Based on the polyphasic analysis presented in this study, we suggest that strain SH-1T represents a novel genus and species in the family Rhodobacteraceae, for which the name Pukyongiella litopenaei gen. nov., sp. nov. is proposed. The type strain of Pukyongiella litopenaei is SH-1T (=KCTC 62276T=MCCC 1K04072T).
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Penaeidae
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Tracto Gastrointestinal
/
Rhodobacteraceae
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2020
Tipo del documento:
Article