Adipose tissue in health and disease through the lens of its building blocks.
Sci Rep
; 10(1): 10433, 2020 06 26.
Article
en En
| MEDLINE
| ID: mdl-32591560
ABSTRACT
Understanding adipose tissue cellular heterogeneity and homeostasis is essential to comprehend the cell type dynamics in metabolic diseases. Cellular subpopulations in the adipose tissue have been related to disease development, but efforts towards characterizing the adipose tissue cell type composition are limited. Here, we identify the cell type composition of the adipose tissue by using gene expression deconvolution of large amounts of publicly available transcriptomics level data. The proposed approach allows to present a comprehensive study of adipose tissue cell type composition, determining the relative amounts of 21 different cell types in 1282 adipose tissue samples detailing differences across four adipose tissue depots, between genders, across ranges of BMI and in different stages of type-2 diabetes. We compare our results to previous marker-based studies by conducting a literature review of adipose tissue cell type composition and propose candidate cellular markers to distinguish different cell types within the adipose tissue. This analysis reveals gender-specific differences in CD4+ and CD8+ T cell subsets; identifies adipose tissue as rich source of multipotent stem/stromal cells; and highlights a strongly increased immune cell content in epicardial and pericardial adipose tissue compared to subcutaneous and omental depots. Overall, this systematic analysis provides comprehensive insights into adipose tissue cell-type heterogeneity in health and disease.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tejido Adiposo
/
Adipocitos
/
Obesidad
Límite:
Humans
Idioma:
En
Revista:
Sci Rep
Año:
2020
Tipo del documento:
Article
País de afiliación:
Países Bajos