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Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles.
Liu, Yuan; Wu, Sherry; Koo, Yeonjong; Yang, An; Dai, Yanwan; Khant, Htet; Osman, Samantha R; Chowdhury, Mamur; Wei, Haichao; Li, Yang; Court, Karem; Hwang, Elaine; Wen, Yunfei; Dasari, Santosh K; Nguyen, Michael; Tang, E Chia-Cheng; Chehab, E Wassim; de Val, Natalia; Braam, Janet; Sood, Anil K.
Afiliación
  • Liu Y; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX; BioSciences, Rice University, Houston, TX. Electronic address: yliu32@mdanderson.org.
  • Wu S; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: sherry.wu@uq.edu.au.
  • Koo Y; BioSciences, Rice University, Houston, TX. Electronic address: yeonjong@gmail.com.
  • Yang A; BioSciences, Rice University, Houston, TX; State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, People's Republic of China. Electronic address: anyang@ibcas.ac.cn.
  • Dai Y; BioSciences, Rice University, Houston, TX. Electronic address: Yanwan.Dai@rice.edu.
  • Khant H; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, Frederick, MD; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Inc., Frederick, MD. Electronic address: htet.khant@nih.gov.
  • Osman SR; BioSciences, Rice University, Houston, TX. Electronic address: sro3@rice.edu.
  • Chowdhury M; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: MAChowdhury@mdanderson.org.
  • Wei H; The Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX. Electronic address: Haichao.Wei@uth.tmc.edu.
  • Li Y; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: YLi52@mdanderson.org.
  • Court K; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: karemcourt@gmail.com.
  • Hwang E; BioSciences, Rice University, Houston, TX. Electronic address: esh3@rice.edu.
  • Wen Y; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: YWen2@mdanderson.org.
  • Dasari SK; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: SKDasari@mdanderson.org.
  • Nguyen M; BioSciences, Rice University, Houston, TX. Electronic address: mhn2@rice.edu.
  • Tang EC; BioSciences, Rice University, Houston, TX. Electronic address: ct44@rice.edu.
  • Chehab EW; BioSciences, Rice University, Houston, TX. Electronic address: ewchehab@rice.edu.
  • de Val N; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, Frederick, MD; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Inc., Frederick, MD. Electronic address: natalia.devalalda@nih.gov.
  • Braam J; BioSciences, Rice University, Houston, TX. Electronic address: Braam@rice.edu.
  • Sood AK; Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: asood@mdanderson.org.
Nanomedicine ; 29: 102271, 2020 10.
Article en En | MEDLINE | ID: mdl-32702466
ABSTRACT
Mammalian small extracellular vesicles (sEVs) can deliver diverse molecules to target cells. However, they are difficult to obtain in large quantities and can activate host immune responses. Plant-derived vesicles may help to overcome these challenges. We optimized isolation methods for two types of plant vesicles, nanovesicles from disrupted leaf and sEVs from the extracellular apoplastic space of Arabidopsis thaliana. Both preparations yielded intact vesicles of uniform size, and a mean membrane charge of approximately -25 mV. We also demonstrated applicability of these preparative methods using Brassicaceae vegetables. Proteomic analysis of a subset of vesicles with a density of 1.1-1.19 g mL-1 sheds light on the likely cellular origin and complexity of the vesicles. Both leaf nanovesicles and sEVs were taken up by cancer cells, with sEVs showing an approximately three-fold higher efficiency compared to leaf nanovesicles. These results support the potential of plant-derived vesicles as vehicles for therapeutic delivery.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Arabidopsis / Hojas de la Planta / Vesículas Extracelulares Límite: Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Arabidopsis / Hojas de la Planta / Vesículas Extracelulares Límite: Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article