Inter-laboratory study of an optimised peptide mapping workflow using automated trypsin digestion for monitoring monoclonal antibody product quality attributes.

Millán-Martín, Silvia; Jakes, Craig; Carillo, Sara; Buchanan, Tom; Guender, Marc; Kristensen, Dan Bach; Sloth, Trine Meiborg; Ørgaard, Martin; Cook, Ken; Bones, Jonathan

Millán-Martín, Silvia; Jakes, Craig; Carillo, Sara; Buchanan, Tom; Guender, Marc; Kristensen, Dan Bach; Sloth, Trine Meiborg; Ørgaard, Martin; Cook, Ken; Bones, Jonathan.

Afiliación

Millán-Martín S; Characterisation and Comparability Laboratory, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co., Dublin, A94 X099, Ireland.
Jakes C; Characterisation and Comparability Laboratory, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co., Dublin, A94 X099, Ireland.
Carillo S; School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland.
Buchanan T; Characterisation and Comparability Laboratory, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co., Dublin, A94 X099, Ireland.
Guender M; Thermo Fisher Scientific, Tudor Rd, Runcorn, WA7 1TA, UK.
Kristensen DB; Thermo Fisher Scientific, Reinach TechCenter, Neuhofstrasse 11, 4153, Basel, Switzerland.
Sloth TM; Symphogen, Pederstrupvej 93, 2750, Ballerup, Denmark.
Ørgaard M; Symphogen, Pederstrupvej 93, 2750, Ballerup, Denmark.
Cook K; Symphogen, Pederstrupvej 93, 2750, Ballerup, Denmark.
Bones J; Thermo Fisher Scientific, Stafford House, 1 Boundary Park, Hemel Hempstead, HP2 7GE, UK.

Anal Bioanal Chem ; 412(25): 6833-6848, 2020 Oct.

Article en En | MEDLINE | ID: mdl-32710279

Asunto(s)

Anticuerpos Monoclonales/química; Mapeo Peptídico/métodos; Tripsina/química; Anticuerpos Monoclonales/inmunología; Automatización; Cromatografía Liquida/métodos; Espectrometría de Masas en Tándem/métodos

Palabras clave

Inter-laboratory study; Method transferability; Monoclonal antibody; Peptide mapping; Post-translational modifications (PTMs); Trypsin digestion

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mapeo Peptídico / Tripsina / Anticuerpos Monoclonales Idioma: En Revista: Anal Bioanal Chem Año: 2020 Tipo del documento: Article País de afiliación: Irlanda

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