Dexamethasone primes adipocyte precursor cells for differentiation by enhancing adipogenic competency.
Life Sci
; 261: 118363, 2020 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-32861797
ABSTRACT
AIM:
Dexamethasone (DXM) is a synthetic glucocorticoid whose effects in early and terminal adipogenesis have been addressed. In this study, we evaluated if DXM affects adipocyte precursor cells (APCs), priming them for further adipogenic differentiation. For this purpose, we analyzed APCs number and competency after DXM treatment. MATERIALS ANDMETHODS:
Adult male rats were injected for 2 or 7â¯days with either DXM (30⯵g/kg of weight, sc.) or vehicle. Stromal vascular fraction (SVF) cells from retroperitoneal adipose tissue (RPAT) were isolated to quantify APCs by flow cytometry (CD34+/CD45-/CD31-). Also, expression of competency markers (PPARγ2 and Zfp423) was assessed. Additionally, SVF cells from control rats were incubated with DXM (0.25⯵M) alone or combined with a mineralocorticoid receptor (MR) antagonist (Spironolactone 10⯵M) and/or a glucocorticoid receptor (GR) antagonist (RU486 1⯵M) to assess APCs competency and adipocyte differentiation. KEYFINDINGS:
APCs from 2â¯days DXM-treated rats showed increased expression of PPARγ2 and Zfp423 (competency markers), but did not affect APCs percentage by FACS analysis (CD34+/CD45-/CD31-). Additionally, we found that DXM treatment in SVF also increased APCs competency in vitro, predisposing APCs to further adipocyte differentiation. These effects on APCs were abrogated only when both, MR and GR, were blocked.SIGNIFICANCE:
Overall, our results suggest that DXM primes APCs for differentiation mainly by enhancing Zfp423 and PPARγ2 expressions. Also, we showed that the inhibition of MR and GR was necessary for the complete abolishment of DXM effects.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Dexametasona
/
Adipocitos
/
Adipogénesis
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
2020
Tipo del documento:
Article
País de afiliación:
Argentina