Sortase A-Inhibitory Coumarins from the Folk Medicinal Plant Poncirus trifoliata.
J Nat Prod
; 83(10): 3004-3011, 2020 10 23.
Article
en En
| MEDLINE
| ID: mdl-32996318
ABSTRACT
Thirteen coumarins (1-13), including five new compounds (1-5), were isolated from the folk medicinal plant Poncirus trifoliata. Combined spectroscopic analyses revealed that coumarins 1-4 are bis-isoprenylated coumarins with diverse oxidation patterns, while 5 is an enantiomeric di-isoprenylated coumarin. The absolute configurations of the stereogenic centers in the isoprenyl chains were assigned through MTPA and MPA methods, and those of the known compounds triphasiol (6) and ponciol (7) were also assigned using similar methods. These coumarins inhibited significantly Staphylococcus aureus-derived sortase A (SrtA), a transpeptidase responsible for anchoring surface proteins to the peptidoglycan cell wall in Gram-positive bacteria. The present results obtained indicated that the bioactivity and underlying mechanism of action of these coumarins are associated with the inhibition of SrtA-mediated S. aureus adhesion to eukaryotic cell matrix proteins including fibrinogen and fibronectin, thus potentially serving as SrtA inhibitors.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plantas Medicinales
/
Proteínas Bacterianas
/
Aminoaciltransferasas
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Cumarinas
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Poncirus
Idioma:
En
Revista:
J Nat Prod
Año:
2020
Tipo del documento:
Article