[Study on changes of coagulation state, microthrombus and vascular density in the model of iron accumulation osteoporosis].

OBJECTIVE: To understand changes of coagulation state, microthrombus, microvascular bed and bone density in the osteoporosis model of iron accumulation, and explore the influence of iron accumulation in aspects of osteoporosis on coagulation function and blood vessels. METHODS: Tewnty-four male SPF SD rats aged 6 months were selected, which with the average body weight (250±20) g, which were divided into control group and iron accumulation group according to random number table, 12 rats in each group. Iron accumulation group was intervened by intraperitoneal injection of ferric ammonium citrate 90 mg / kg, and control group was intraperitoneally injected with equal volume of normal saline, twice a week for 9 weeks. After intervention, serum ferritin, coagulation function, microthrombus, vascular density, and three-dimensional morphological reconstruction and spatial structure parameters of the distal femur trabeculae were measured and statistically analyzed. RESULTS: Serum ferritin of iron accumulation group (136.36±35.41) µg / L was higher than control group (68.44±16.86) µg / L(P<0.05). Bone mineral density (BMD) of iron accumulation group (0.167±0.024) g / cm3 was lower than control group(0.400±0.030)g / cm3. Fibrinogen of iron accumulation group (2.03±0.13) g / L was increased than that of control group (1.78±0.46) g / L, D-dimer contents of iron accumulation group (534.95±31.81) ng /ml was increased than that of control group (329.02±84.99) ng /ml, while thrombin time (39.64±2.18) s and prothrombin time(8.70±0.39) s of iron accumulation group were shorter than that of control group (44.92±2.98) s, (9.44±0.49) s (P<0.05). After ink staining, microvessel density in iron accumulation group (17.46±2.07)% was significantly reducedcompared with that of control group(23.81±2.98)%(P<0.05). HE and MSB staining which showed microthrombus in bone marrow of iron accumulation rats, as well as microthrombus in myocardium. CONCLUSION: In the osteoporosis model with the influence of iron accumulation, iron accumulation had a significant influence on the coagulation function, and the blood was relatively hypercoagulable. The bone vascular bed uas reduced, and there were microthrombus in the bone marrow. Hypercoagulable state of blood and formation of microthrombi may be important factors influencing the occurrence of iron accumulation osteoporosis.