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The smfBox is an open-source platform for single-molecule FRET.
Ambrose, Benjamin; Baxter, James M; Cully, John; Willmott, Matthew; Steele, Elliot M; Bateman, Benji C; Martin-Fernandez, Marisa L; Cadby, Ashley; Shewring, Jonathan; Aaldering, Marleen; Craggs, Timothy D.
Afiliación
  • Ambrose B; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Baxter JM; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Cully J; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Willmott M; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Steele EM; Department of Physics, University of Sheffield, Sheffield, UK.
  • Bateman BC; Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Oxford, UK.
  • Martin-Fernandez ML; Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Oxford, UK.
  • Cadby A; Department of Physics, University of Sheffield, Sheffield, UK.
  • Shewring J; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Aaldering M; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.
  • Craggs TD; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK. t.craggs@sheffield.ac.uk.
Nat Commun ; 11(1): 5641, 2020 11 06.
Article en En | MEDLINE | ID: mdl-33159061
ABSTRACT
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido