MicroRNA1455p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells.
Mol Med Rep
; 22(6): 5282-5292, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-33174029
ABSTRACT
MicroRNAs (miRs) can affect the progression of cervical cancer (CC). The present study investigated the function of miR1455p in CC and demonstrated its association with fascin (FSCN1). The expression levels of miR1455p in CC tissues and cell lines were analyzed using reverse transcriptionquantitative PCR, and its direct targets were explored using a luciferase reporter assay. The viability, migration and invasion of HeLa cells transfected with small interfering FSCN1 or with miR1455p mimics and inhibitors were analyzed using Cell Counting Kit8 and Transwell assays. The expression levels of FSCN1 mRNA and protein were investigated using reverse transcription PCR and western blotting. miR1455p was downregulated in CC tissues and cell lines. Moreover, overexpression of miR1455p inhibited the migration, invasion and viability of HeLa cells. miR1455p directly targeted FSCN1, which regulated the suppressive functions of miR1455p in CC cells. Overall, miR1455p is a tumor suppressor gene and a promising target for CC treatment.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Neoplasias del Cuello Uterino
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MicroARNs
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Proteínas de Microfilamentos
Límite:
Adult
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Female
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Humans
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Middle aged
País/Región como asunto:
Asia
Idioma:
En
Revista:
Mol Med Rep
Año:
2020
Tipo del documento:
Article