Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells.

Pijlman, Gorben P; Grose, Carissa; Hick, Tessy A H; Breukink, Herman E; van den Braak, Robin; Abbo, Sandra R; Geertsema, Corinne; van Oers, Monique M; Martens, Dirk E; Esposito, Dominic

Pijlman, Gorben P; Grose, Carissa; Hick, Tessy A H; Breukink, Herman E; van den Braak, Robin; Abbo, Sandra R; Geertsema, Corinne; van Oers, Monique M; Martens, Dirk E; Esposito, Dominic.

Afiliación

Pijlman GP; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Grose C; Protein Expression Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc. PO Box B, Frederick, MD 21702, USA.
Hick TAH; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Breukink HE; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
van den Braak R; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Abbo SR; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Geertsema C; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
van Oers MM; Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Martens DE; Bioprocess Engineering, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
Esposito D; Protein Expression Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc. PO Box B, Frederick, MD 21702, USA.

Viruses ; 12(12)2020 12 16.

Article en En | MEDLINE | ID: mdl-33339324

Asunto(s)

Baculoviridae/genética; Genoma Viral; Inestabilidad Genómica; Recombinación Homóloga; Mutagénesis Insercional; Proteínas Recombinantes/genética; Transgenes; Animales; Reactores Biológicos; Línea Celular; Virus Chikungunya/inmunología; Ingeniería Genética; Vectores Genéticos/genética; Insectos; Células Sf9; Vacunas de Partículas Similares a Virus/inmunología

Palabras clave

Tn7; VLPs; bacmid; baculovirus; bioreactor; chikungunya virus; genome stability; insect cells; protein expression

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Baculoviridae / Mutagénesis Insercional / Genoma Viral / Transgenes / Inestabilidad Genómica / Recombinación Homóloga Límite: Animals Idioma: En Revista: Viruses Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos

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