Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
STAR Protoc
; 1(3): 100173, 2020 12 18.
Article
en En
| MEDLINE
| ID: mdl-33377067
ABSTRACT
Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells. This will be useful for mechanistic studies of viral and cellular factors driving flavivirus RO biogenesis. For complete details on the use and execution of this protocol, please refer to Cerikan et al. (2020).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
Orgánulos
/
Técnicas Citológicas
/
Flavivirus
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania