Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection of Respiratory Pathogens Including SARS-CoV-2.

Wang, Yu; Liu, Donglan; Lin, Huihuang; Chen, Dingbin; Sun, Jing; Xie, Yusang; Wang, Xinjie; Ma, Peixiang; Nie, Yan; Mei, Hong; Zhao, Baihui; Huang, Xingxu; Jiang, Ge; Jiang, Xianxing; Qu, Jieming; Zhao, Jincun; Liu, Jia

Wang, Yu; Liu, Donglan; Lin, Huihuang; Chen, Dingbin; Sun, Jing; Xie, Yusang; Wang, Xinjie; Ma, Peixiang; Nie, Yan; Mei, Hong; Zhao, Baihui; Huang, Xingxu; Jiang, Ge; Jiang, Xianxing; Qu, Jieming; Zhao, Jincun; Liu, Jia.

Afiliación

Wang Y; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Liu D; Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510006, China.
Lin H; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China.
Chen D; Department of Pulmonary and Critical Care Medicine, Ruijin Hospital and Institutes of Respiratory Diseases, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
Sun J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China.
Xie Y; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China.
Wang X; Department of Pulmonary and Critical Care Medicine, Ruijin Hospital and Institutes of Respiratory Diseases, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
Ma P; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Nie Y; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Mei H; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Zhao B; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Huang X; Shanghai BioGerm Medical Biotechnology Co., Ltd, Shanghai 201318, China.
Jiang G; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Jiang X; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
Qu J; Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510006, China.
Zhao J; Department of Pulmonary and Critical Care Medicine, Ruijin Hospital and Institutes of Respiratory Diseases, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
Liu J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China.

ACS Chem Biol ; 16(3): 491-500, 2021 03 19.

Article en En | MEDLINE | ID: mdl-33586431

Asunto(s)

Proteínas Bacterianas/genética; Prueba de Ácido Nucleico para COVID-19/métodos; COVID-19/diagnóstico; Proteínas Asociadas a CRISPR/genética; Sistemas CRISPR-Cas; Endodesoxirribonucleasas/genética; ARN Viral/análisis; SARS-CoV-2/química; Adenoviridae/química; Animales; COVID-19/genética; Colorantes Fluorescentes/química; Humanos; Límite de Detección; Ratones Endogámicos BALB C; Técnicas de Amplificación de Ácido Nucleico; Sondas ARN/genética; ARN Viral/genética; Manejo de Especímenes; Espectrometría de Fluorescencia

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / ARN Viral / Endodesoxirribonucleasas / Proteínas Asociadas a CRISPR / Sistemas CRISPR-Cas / Prueba de Ácido Nucleico para COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: ACS Chem Biol Año: 2021 Tipo del documento: Article País de afiliación: China

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