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Impact of Bicarbonate on PBP2a Production, Maturation, and Functionality in Methicillin-Resistant Staphylococcus aureus (MRSA).
Ersoy, Selvi C; Chambers, Henry F; Proctor, Richard A; Rosato, Adriana E; Mishra, Nagendra N; Xiong, Yan Q; Bayer, Arnold S.
Afiliación
  • Ersoy SC; The Lundquist Institute, Torrance, CA, USA.
  • Chambers HF; UCSF School of Medicine, San Francisco, CA, USA.
  • Proctor RA; Departments of Medicine and Medical Microbiology/Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
  • Rosato AE; Department of Pathology, Riverside University Health Systems, Riverside, CA, USA.
  • Mishra NN; The Lundquist Institute, Torrance, CA, USA.
  • Xiong YQ; Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
  • Bayer AS; The Lundquist Institute, Torrance, CA, USA.
Article en En | MEDLINE | ID: mdl-33649115
Certain methicillin-resistant Staphylococcus aureus (MRSA) strains exhibit ß-lactam-susceptibility in vitro, ex vivo and in vivo in the presence of NaHCO3 (NaHCO3-responsive MRSA). Herein, we investigate the impact of NaHCO3 on factors required for PBP2a functionality. Prototype NaHCO3-responsive and -nonresponsive MRSA strains (as defined in vitro) were assessed for the impact of NaHCO3 on: expression of genes involved in PBP2a production-maturation pathways (mecA, blaZ, pbp4, vraSR, prsA, sigB, and floA); membrane PBP2a and PrsA protein content; and membrane carotenoid content. Following NaHCO3 exposure in NaHCO3-responsive (vs - nonresponsive) MRSA, there was significantly reduced expression of: i) mecA and blaZ; ii) the vraSR-prsA gene axis; and iii) pbp4 Carotenoid production was reduced, while floA expression was increased by NaHCO3 exposure in all MRSA strains. This work underscores the distinct regulatory impact of NaHCO3 on a cadre of genes encoding factors required for maintenance of the MRSA phenotype through PBP2a functionality and maturation.

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Antimicrob Agents Chemother Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Antimicrob Agents Chemother Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos