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Neutralizing IFNL3 Autoantibodies in Severe COVID-19 Identified Using Molecular Indexing of Proteins by Self-Assembly.
Credle, Joel J; Gunn, Jonathan; Sangkhapreecha, Puwanat; Monaco, Daniel R; Zheng, Xuwen Alice; Tsai, Hung-Ji; Wilbon, Azaan; Morgenlander, William R; Dong, Yi; Jayaraman, Sahana; Tosi, Lorenzo; Parekkadan, Biju; Baer, Alan N; Roederer, Mario; Bloch, Evan M; Tobian, Aaron A R; Zyskind, Israel; Silverberg, Jonathan I; Rosenberg, Avi Z; Cox, Andrea L; Lloyd, Tom; Mammen, Andrew L; Larman, H Benjamin.
Afiliación
  • Credle JJ; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Gunn J; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Sangkhapreecha P; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Monaco DR; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Zheng XA; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Tsai HJ; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston; Birmingham, United Kingdom.
  • Wilbon A; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Morgenlander WR; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Dong Y; Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Jayaraman S; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Tosi L; Department of Biomedical Engineering, Rutgers University; Piscataway, NJ, USA.
  • Parekkadan B; Department of Biomedical Engineering, Rutgers University; Piscataway, NJ, USA.
  • Baer AN; Division of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Roederer M; ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH; Bethesda, MD, USA.
  • Bloch EM; Division of Transfusion Medicine, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Tobian AAR; Division of Transfusion Medicine, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Zyskind I; Department of Pediatrics, NYU Langone Medical Center, New York, NY and Maimonides Medical Center; Brooklyn, NY, USA.
  • Silverberg JI; Department of Dermatology, George Washington University School of Medicine and Health Sciences; Washington, DC, USA.
  • Rosenberg AZ; Division of Transfusion Medicine, Department of Pathology, Johns Hopkins University; Baltimore, MD, USA.
  • Cox AL; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University; Baltimore, MD, USA.
  • Lloyd T; Departments of Neurology and Neuroscience, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Mammen AL; Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH; Bethesda, MD, USA and Departments of Neurology and Medicine, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
  • Larman HB; Institute for Cell Engineering, Division of Immunology, Department of Pathology, Johns Hopkins University School of Medicine; Baltimore, MD, USA.
bioRxiv ; 2021 Mar 03.
Article en En | MEDLINE | ID: mdl-33688651
ABSTRACT
Unbiased antibody profiling can identify the targets of an immune reaction. A number of likely pathogenic autoreactive antibodies have been associated with life-threatening SARS-CoV-2 infection; yet, many additional autoantibodies likely remain unknown. Here we present Molecular Indexing of Proteins by Self Assembly (MIPSA), a technique that produces ORFeome-scale libraries of proteins covalently coupled to uniquely identifying DNA barcodes for analysis by sequencing. We used MIPSA to profile circulating autoantibodies from 55 patients with severe COVID-19 against 11,076 DNA-barcoded proteins of the human ORFeome library. MIPSA identified previously known autoreactivities, and also detected undescribed neutralizing interferon lambda 3 (IFN-λ3) autoantibodies. At-risk individuals with anti- IFN-λ3 antibodies may benefit from interferon supplementation therapies, such as those currently undergoing clinical evaluation.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos