Transcriptional profiling reveals the transcription factor networks regulating the survival of striatal neurons.
Cell Death Dis
; 12(3): 262, 2021 03 12.
Article
en En
| MEDLINE
| ID: mdl-33712552
ABSTRACT
The striatum is structurally highly diverse, and its organ functionality critically depends on normal embryonic development. Although several studies have been conducted on the gene functional changes that occur during striatal development, a system-wide analysis of the underlying molecular changes is lacking. Here, we present a comprehensive transcriptome profile that allows us to explore the trajectory of striatal development and identify the correlation between the striatal development and Huntington's disease (HD). Furthermore, we applied an integrative transcriptomic profiling approach based on machine learning to systematically map a global landscape of 277 transcription factor (TF) networks. Most of these TF networks are linked to biological processes, and some unannotated genes provide information about the corresponding mechanisms. For example, we found that the Meis2 and Six3 were crucial for the survival of striatal neurons, which were verified using conditional knockout (CKO) mice. Finally, we used RNA-Seq to speculate their downstream targets.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Apoptosis
/
Enfermedad de Huntington
/
Cuerpo Estriado
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Perfilación de la Expresión Génica
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Redes Reguladoras de Genes
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Transcriptoma
Tipo de estudio:
Observational_studies
/
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Cell Death Dis
Año:
2021
Tipo del documento:
Article