Amazonian mammal monitoring using aquatic environmental DNA.
Mol Ecol Resour
; 21(6): 1875-1888, 2021 Aug.
Article
en En
| MEDLINE
| ID: mdl-33787010
ABSTRACT
Environmental DNA (eDNA) metabarcoding has emerged as one of the most efficient methods to assess aquatic species presence. While the method can in theory be used to investigate nonaquatic fauna, its development for inventorying semi-aquatic and terrestrial fauna is still at an early stage. Here we investigated the potential of aquatic eDNA metabarcoding for inventorying mammals in Neotropical environments, be they aquatic, semi-aquatic or terrestrial. We collected aquatic eDNA in 96 sites distributed along three Guianese watersheds and compared our inventories to expected species distributions and field observations derived from line transects located throughout French Guiana. Species occurrences and emblematic mammalian fauna richness patterns were consistent with the expected distribution of fauna and our results revealed that aquatic eDNA metabarcoding brings additional data to line transect samples for diurnal nonaquatic (terrestrial and arboreal) species. Aquatic eDNA also provided data on species not detectable in line transect surveys such as semi-aquatic, aquatic and nocturnal terrestrial and arboreal species. Although the application of eDNA to inventory mammals still needs some developments to optimize sampling efficiency, it can now be used as a complement to traditional surveys.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Agua
/
ADN Ambiental
/
Mamíferos
Tipo de estudio:
Qualitative_research
Límite:
Animals
País/Región como asunto:
America do sul
/
Guyana francesa
Idioma:
En
Revista:
Mol Ecol Resour
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia