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Theoretical Considerations for Next-Generation Proteomics.
Palmblad, Magnus.
Afiliación
  • Palmblad M; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2300 RC, The Netherlands.
J Proteome Res ; 20(6): 3395-3399, 2021 06 04.
Article en En | MEDLINE | ID: mdl-33904308
While mass spectrometry still dominates proteomics research, alternative and potentially disruptive, next-generation technologies are receiving increased investment and attention. Most of these technologies aim at the sequencing of single peptide or protein molecules, typically labeling or otherwise distinguishing a subset of the proteinogenic amino acids. This note considers some theoretical aspects of these future technologies from a bottom-up proteomics viewpoint, including the ability to uniquely identify human proteins as a function of which and how many amino acids can be read, enzymatic efficiency, and the maximum read length. This is done through simulations under ideal and non-ideal conditions to set benchmarks for what may be achievable with future single-molecule sequencing technology. The simulations reveal, among other observations, that the best choice of reading N amino acids performs similarly to the average choice of N+1 amino acids, and that the discrimination power of the amino acids scales with their frequency in the proteome. The simulations are agnostic with respect to the next-generation proteomics platform, and the results and conclusions should therefore be applicable to any single-molecule partial peptide sequencing technology.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteoma / Proteómica Límite: Humans Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteoma / Proteómica Límite: Humans Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos