Enhancing the Photocatalytic Conversion of Pt(IV) Substrates by Flavoprotein Engineering.
J Phys Chem Lett
; 12(19): 4504-4508, 2021 May 20.
Article
en En
| MEDLINE
| ID: mdl-33960797
ABSTRACT
Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site-directed mutagenesis on the mini singlet oxygen generator (mSOG) to modulate the photocatalytic activity of this flavoprotein toward two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited-state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein toward metal-containing substrates.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Platino (Metal)
/
Ingeniería de Proteínas
/
Complejos de Coordinación
/
Flavoproteínas
Idioma:
En
Revista:
J Phys Chem Lett
Año:
2021
Tipo del documento:
Article
País de afiliación:
España