AB/PAS, Alcian blue/Periodic acid Schiff; BLOQ, below limits of quantitation; BSA, bovine serum albumin; BrdU, 5-bromo-2'-deoxyuridine; CBF, ciliary beat frequency; CC3, cleaved caspase-3; CO, Carbon monoxide; CS, cigarette smoke; CSE, cigarette smoke exposure; Cell death; Cell proliferation; Cigarette smoke; DAB, 3,3'-diaminobenzidine; FTC/ISO, Federal Trade Commission/International Standard Organization; GSD, geometric standard deviation; H&E, Hematoxylin and Eosin; HIER, heat-induced antigen retrieval; HPF, high power field; MCC, mucociliary clearance; MMAD, Mass median aerodynamic diameter; Mucus production; Murine larynx; NMR, nicotine metabolite ratio; OECD, organization for economic co-operation and development; PAHs, polycyclic aromatic hydrocarbons; RE, respiratory epithelium; REV, reversibility; ROS, reactive oxygen species; SCIREQ, Scientific Respiratory Equipment Inc; SEM, scanning electron microscopy; SSE, stratified squamous epithelium; SWGTOX, Scientific Working Group for Forensic Toxicology; Surface topography; TBST, tris-buffered saline-tween 20; TPM, total particulate matter; TSNA, tobacco-specific nitrosamines; UPLC-MS/MS, ultra-performance liquid chromatography-tandem mass spectrometer; VF, vocal fold; VSC, veterinary service center