Optogenetic Control of the Canonical Wnt Signaling Pathway During Xenopus laevis Embryonic Development.
J Mol Biol
; 433(18): 167050, 2021 09 03.
Article
en En
| MEDLINE
| ID: mdl-34019868
ABSTRACT
Optogenetics uses light-inducible protein-protein interactions to precisely control the timing, localization, and intensity of signaling activity. The precise spatial and temporal resolution of this emerging technology has proven extremely attractive to the study of embryonic development, a program faithfully replicated to form the same organism from a single cell. We have previously performed a comparative study for optogenetic activation of receptor tyrosine kinases, where we found that the cytoplasm-to-membrane translocation-based optogenetic systems outperform the membrane-anchored dimerization systems in activating the receptor tyrosine kinase signaling in live Xenopus embryos. Here, we determine if this engineering strategy can be generalized to other signaling pathways involving membrane-bound receptors. As a proof of concept, we demonstrate that the cytoplasm-to-membrane translocation of the low-density lipoprotein receptor-related protein-6 (LRP6), a membrane-bound coreceptor for the canonical Wnt pathway, triggers Wnt activity. Optogenetic activation of LRP6 leads to axis duplication in developing Xenopus embryos, indicating that the cytoplasm-to-membrane translocation of the membrane-bound receptor could be a generalizable strategy for the construction of optogenetic systems.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regulación del Desarrollo de la Expresión Génica
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Citoplasma
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Proteínas de Xenopus
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Embrión no Mamífero
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Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad
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Vía de Señalización Wnt
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Optogenética
Límite:
Animals
Idioma:
En
Revista:
J Mol Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos