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Respiratory Tract Explant Infection Dynamics of Influenza A Virus in California Sea Lions, Northern Elephant Seals, and Rhesus Macaques.
Liu, Hongwei; Plancarte, Magdalena; Ball, Erin E; Weiss, Christopher M; Gonzales-Viera, Omar; Holcomb, Karen; Ma, Zhong-Min; Allen, A Mark; Reader, J Rachel; Duignan, Pádraig J; Halaska, Barbie; Khan, Zenab; Kriti, Divya; Dutta, Jayeeta; van Bakel, Harm; Jackson, Kenneth; Pesavento, Patricia A; Boyce, Walter M; Coffey, Lark L.
Afiliación
  • Liu H; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Plancarte M; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Ball EE; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Weiss CM; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Gonzales-Viera O; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Holcomb K; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Ma ZM; University of California, Davis, California National Primate Research Center, Davis, California, USA.
  • Allen AM; University of California, Davis, California National Primate Research Center, Davis, California, USA.
  • Reader JR; University of California, Davis, California National Primate Research Center, Davis, California, USA.
  • Duignan PJ; The Marine Mammal Center, Sausalito, California, USA.
  • Halaska B; The Marine Mammal Center, Sausalito, California, USA.
  • Khan Z; Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Kriti D; Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Dutta J; Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • van Bakel H; Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Jackson K; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Pesavento PA; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Boyce WM; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
  • Coffey LL; University of California, Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, Davis, California, USA.
J Virol ; 95(16): e0040321, 2021 07 26.
Article en En | MEDLINE | ID: mdl-34037419
ABSTRACT
To understand susceptibility of wild California sea lions and Northern elephant seals to influenza A virus (IAV), we developed an ex vivo respiratory explant model and used it to compare infection kinetics for multiple IAV subtypes. We first established the approach using explants from colonized rhesus macaques, a model for human IAV. Trachea, bronchi, and lungs from 11 California sea lions, 2 Northern elephant seals, and 10 rhesus macaques were inoculated within 24 h postmortem with 6 strains representing 4 IAV subtypes. Explants from the 3 species showed similar IAV infection kinetics, with peak viral titers 48 to 72 h post-inoculation that increased by 2 to 4 log10 PFU/explant relative to the inoculum. Immunohistochemistry localized IAV infection to apical epithelial cells. These results demonstrate that respiratory tissue explants from wild marine mammals support IAV infection. In the absence of the ability to perform experimental infections of marine mammals, this ex vivo culture of respiratory tissues mirrors the in vivo environment and serves as a tool to study IAV susceptibility, host range, and tissue tropism. IMPORTANCE Although influenza A virus can infect marine mammals, a dearth of marine mammal cell lines and ethical and logistical challenges prohibiting experimental infections of living marine mammals mean that little is known about IAV infection kinetics in these species. We circumvented these limitations by adapting a respiratory tract explant model first to establish the approach with rhesus macaques and then for use with explants from wild marine mammals euthanized for nonrespiratory medical conditions. We observed that multiple strains representing 4 IAV subtypes infected trachea, bronchi, and lungs of macaques and marine mammals with variable peak titers and kinetics. This ex vivo model can define infection dynamics for IAV in marine mammals. Further, use of explants from animals euthanized for other reasons reduces use of animals in research.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Virus de la Influenza A / Infecciones del Sistema Respiratorio / Infecciones por Orthomyxoviridae Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Virol Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Virus de la Influenza A / Infecciones del Sistema Respiratorio / Infecciones por Orthomyxoviridae Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Virol Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos