The Retinal Ganglion Cell Response to Blast-Mediated Traumatic Brain Injury Is Genetic Background Dependent.
Invest Ophthalmol Vis Sci
; 62(7): 13, 2021 06 01.
Article
en En
| MEDLINE
| ID: mdl-34106210
ABSTRACT
Purpose:
The purpose of this study was to examine the influence of genetic background on the retinal ganglion cell (RGC) response to blast-mediated traumatic brain injury (TBI) in Jackson Diversity Outbred (JDO), C57BL/6J and BALB/cByJ mice.Methods:
Mice were subject to one blast injury of 137 kPa. RGC structure was analyzed by optical coherence tomography (OCT), function by the pattern electroretinogram (PERG), and histologically using BRN3A antibody staining.Results:
Comparison of the change in each group from baseline for OCT and PERG was performed. There was a significant difference in the JDOΔOCT compared to C57BL/6J mice (P = 0.004), but not compared to BALB/cByJ (P = 0.21). There was a significant difference in the variance of the ΔOCT in JDO compared to both C57BL/6J and BALB/cByJ mice. The baseline PERG amplitude was 20.33 ± 9.32 µV, which decreased an average of -4.14 ± 12.46 µV following TBI. Baseline RGC complex + RNFL thickness was 70.92 ± 4.52 µm, which decreased an average of -1.43 ± 2.88 µm following blast exposure. There was not a significant difference in the ΔPERG between JDO and C57BL/6J (P = 0.13), although the variances of the groups were significantly different. Blast exposure in JDO mice results in a density change of 558.6 ± 440.5 BRN3A-positive RGCs/mm2 (mean ± SD).Conclusions:
The changes in retinal outcomes had greater variance in outbred mice than what has been reported, and largely replicated herein, for inbred mice. These results demonstrate that the RGC response to blast injury is highly dependent upon genetic background.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Retina
/
Células Ganglionares de la Retina
/
Estrés Fisiológico
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Traumatismos por Explosión
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Factor de Transcripción Brn-3A
/
Lesiones Traumáticas del Encéfalo
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos