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A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells.
Funnell, Simon G P; Afrough, Babak; Baczenas, John James; Berry, Neil; Bewley, Kevin R; Bradford, Rebecca; Florence, Clint; Duff, Yann Le; Lewis, Mark; Moriarty, Ryan V; Connor, Shelby L O; Osman, Karen L; Pullan, Steven; Rashid, Sujatha; Richards, Kevin S; Stemple, Kimberly J; Knezevic, Ivana.
Afiliación
  • Funnell SGP; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
  • Afrough B; Quadram Institute Bioscience, Norwich Research Park, Norfolk, UK.
  • Baczenas JJ; World Health Organization, Geneva, Switzerland.
  • Berry N; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
  • Bewley KR; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, USA.
  • Bradford R; Division of Infectious Disease Diagnostics, National Institute for Biological Standards and Control, London, UK.
  • Florence C; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
  • Duff YL; Biodefense and Emerging Infections Resources, NIH, Bethesda, MD, USA.
  • Lewis M; Office of Biodefense, Research Resources and Translational Research, Division of Microbiology and Infectious Diseases, NIAID, NIH, Bethesda, MD, USA.
  • Moriarty RV; Division of Infectious Disease Diagnostics, National Institute for Biological Standards and Control, London, UK.
  • Connor SLO; Office of Biodefense, Research Resources and Translational Research, Division of Microbiology and Infectious Diseases, NIAID, NIH, Bethesda, MD, USA.
  • Osman KL; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, USA.
  • Pullan S; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, USA.
  • Rashid S; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
  • Richards KS; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
  • Stemple KJ; Biodefense and Emerging Infections Resources, NIH, Bethesda, MD, USA.
  • Knezevic I; PHE Porton Down, National Infection Service, Public Health England, Salisbury, Wiltshire, UK.
NPJ Vaccines ; 6(1): 83, 2021 Jun 17.
Article en En | MEDLINE | ID: mdl-34140522
ABSTRACT
An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: NPJ Vaccines Año: 2021 Tipo del documento: Article País de afiliación: Reino Unido
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: NPJ Vaccines Año: 2021 Tipo del documento: Article País de afiliación: Reino Unido