Quantitative phase measurements of human cell nuclei using X-ray ptychography.
J Synchrotron Radiat
; 28(Pt 4): 1166-1173, 2021 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-34212880
ABSTRACT
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Microscopía de Contraste de Fase
Límite:
Humans
Idioma:
En
Revista:
J Synchrotron Radiat
Asunto de la revista:
RADIOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido