ARNSHL, autosomal recessive non-syndromic hearing loss; Actb, Actin beta; BAM, Binary Alignment Map; BWA, Burrows-Wheeler Aligner; C1QTNF9, C1q and TNF related 9; Cx26, Connexin 26; ESRRAP2, Estrogen-Related Receptor Alpha Pseudogene 2; GJB2 gene; GJB2, Gap Junction Protein Beta 2; HHLA1, HERV-H LTR-Associating 1; HL, Hearing loss; KCNQ3, Potassium Voltage-Gated Channel Subfamily Q Member 3; Missense mutation; NGS, next generation sequencing; NSHL, Non-syndromic hearing loss; Non-syndromic hearing loss; PROVEAN, Protein Variation Effect Analyzer; PolyPhen-2, Polymorphism Phenotyping v2; RFLP, restriction fragment length polymorphism; ROH, runs of homozygosity; RT-PCR, reverse transcription PCR; RT-qPCR, quantitative reverse transcription PCR; SAM, Sequence Alignment/Map; SIFT, Sorting Intolerant From Tolerant; SJL, Swiss Jim Lambert; SPATA13, Spermatogenesis Associated 13; ST3GAL1, ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 1; TMEM59, Transmembrane Protein 59; UAE, United Arab Emirates; VariMAT, Variation and Mutation Annotation Toolkit; WES, Whole exome sequencing; Whole exome sequencing; dpSNP, Single Nucleotide Polymorphism Database; gEAR, gene Expression Analysis Resource; gnomAD, genome aggregation database; qPCR, quantitative PCR