Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology.
Open Forum Infect Dis
; 8(8): ofab364, 2021 Aug.
Article
en En
| MEDLINE
| ID: mdl-34409122
ABSTRACT
BACKGROUND:
The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.METHODS:
Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.RESULTS:
The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours.CONCLUSIONS:
We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Open Forum Infect Dis
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania