A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves.
STAR Protoc
; 2(3): 100734, 2021 09 17.
Article
en En
| MEDLINE
| ID: mdl-34430909
ABSTRACT
A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues. For complete details on the use and execution of this protocol, please refer to Yun et al, (2020).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nervio Oftálmico
/
Inmunohistoquímica
/
Córnea
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos