A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization.
Biosensors (Basel)
; 11(8)2021 Aug 06.
Article
en En
| MEDLINE
| ID: mdl-34436067
ABSTRACT
Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 1601 in the dynamic range of 101 to 104 copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 1601 indicate the high clinical applicability of the SWAT.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Hibridación de Ácido Nucleico
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biosensors (Basel)
Año:
2021
Tipo del documento:
Article