Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants.
Proc Natl Acad Sci U S A
; 118(37)2021 09 14.
Article
en En
| MEDLINE
| ID: mdl-34497125
ABSTRACT
Absolute quantification measurements (copies per cell) of peptide major histocompatibility complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; however, existing methods for absolute quantification have critical limitations. Here, we present a platform termed SureQuant-IsoMHC, utilizing a series of pMHC isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine endogenous pMHC concentrations for a panel of 18 tumor antigens. We apply SureQuant-IsoMHC to measure changes in expression of our target panel in a melanoma cell line treated with a MEK inhibitor and translate this approach to estimate antigen concentrations in melanoma tumor biopsies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bencimidazoles
/
Antígenos de Histocompatibilidad Clase I
/
Presentación de Antígeno
/
MAP Quinasa Quinasa 1
/
Melanoma
/
Antígenos de Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2021
Tipo del documento:
Article