Your browser doesn't support javascript.
loading
Renal Denervation Exacerbates LPS- and Antibody-induced Acute Kidney Injury, but Protects from Pyelonephritis in Mice.
Böhner, Alexander M C; Jacob, Alice M; Heuser, Christoph; Stumpf, Natascha E; Effland, Alexander; Abdullah, Zeinab; Meyer-Schwesiger, Catherine; von Vietinghoff, Sibylle; Kurts, Christian.
Afiliación
  • Böhner AMC; Institute for Molecular Medicine and Experimental Immunology, University Hospital of Bonn, Germany.
  • Jacob AM; Department of Radiation Oncology, University Hospital of Bonn, Germany.
  • Heuser C; Institute for Molecular Medicine and Experimental Immunology, University Hospital of Bonn, Germany.
  • Stumpf NE; Institute for Molecular Medicine and Experimental Immunology, University Hospital of Bonn, Germany.
  • Effland A; Institute for Molecular Medicine and Experimental Immunology, University Hospital of Bonn, Germany.
  • Abdullah Z; Institute for Applied Mathematics, University of Bonn, Germany.
  • Meyer-Schwesiger C; Institute for Molecular Medicine and Experimental Immunology, University Hospital of Bonn, Germany.
  • von Vietinghoff S; Institute for Cellular and Integrative Physiology, University Hospital Hamburg, Germany.
  • Kurts C; Nephrology Section, University Hospital of Bonn, Germany.
J Am Soc Nephrol ; 32(10): 2445-2453, 2021 10.
Article en En | MEDLINE | ID: mdl-34599036
BACKGROUND: Renal denervation (RDN) is an invasive intervention to treat drug-resistant arterial hypertension. Its therapeutic value is contentious. Here we examined the effects of RDN on inflammatory and infectious kidney disease models in mice. METHODS: Mice were unilaterally or bilaterally denervated, or sham operated, then three disease models were induced: nephrotoxic nephritis (NTN, a model for crescentic GN), pyelonephritis, and acute endotoxemic kidney injury (as a model for septic kidney injury). Analytical methods included measurement of renal glomerular filtration, proteinuria, flow cytometry of renal immune cells, immunofluorescence microscopy, and three-dimensional imaging of optically cleared kidney tissue by light-sheet fluorescence microscopy followed by algorithmic analysis. RESULTS: Unilateral RDN increased glomerular filtration in denervated kidneys, but decreased it in the contralateral kidneys. In the NTN model, more nephritogenic antibodies were deposited in glomeruli of denervated kidneys, resulting in stronger inflammation and injury in denervated compared with contralateral nondenervated kidneys. Also, intravenously injected LPS increased neutrophil influx and inflammation in the denervated kidneys, both after unilateral and bilateral RDN. When we induced pyelonephritis in bilaterally denervated mice, both kidneys contained less bacteria and neutrophils. In unilaterally denervated mice, pyelonephritis was attenuated and intrarenal neutrophil numbers were lower in the denervated kidneys. The nondenervated contralateral kidneys harbored more bacteria, even compared with sham-operated mice, and showed the strongest influx of neutrophils. CONCLUSIONS: Our data suggest that the increased perfusion and filtration in denervated kidneys can profoundly influence concomitant inflammatory diseases. Renal deposition of circulating nephritic material is higher, and hence antibody- and endotoxin-induced kidney injury was aggravated in mice. Pyelonephritis was attenuated in denervated murine kidneys, because the higher glomerular filtration facilitated better flushing of bacteria with the urine, at the expense of contralateral, nondenervated kidneys after unilateral denervation.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Desnervación Autonómica / Vasoespasmo Coronario / Lesión Renal Aguda / Hipertensión / Nefritis Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: J Am Soc Nephrol Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Desnervación Autonómica / Vasoespasmo Coronario / Lesión Renal Aguda / Hipertensión / Nefritis Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: J Am Soc Nephrol Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Alemania