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Chromosome-level genome assemblies of two cotton-melon aphid Aphis gossypii biotypes unveil mechanisms of host adaption.
Zhang, Shuai; Gao, Xueke; Wang, Li; Jiang, Weili; Su, Honghua; Jing, Tianxing; Cui, Jinjie; Zhang, Lijuan; Yang, Yizhong.
Afiliación
  • Zhang S; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
  • Gao X; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
  • Wang L; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
  • Jiang W; Basic Experimental Teaching Center of Life Sciences, Yangzhou University, Yangzhou, China.
  • Su H; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
  • Jing T; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
  • Cui J; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
  • Zhang L; Department of Entomology, College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
  • Yang Y; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
Mol Ecol Resour ; 22(3): 1120-1134, 2022 Apr.
Article en En | MEDLINE | ID: mdl-34601821
ABSTRACT
The cotton-melon aphid Aphis gossypii is a sap-sucking insect that is considered a serious global pest. The species is distributed over a large geographical range and uses a wide variety of hosts, with some populations being specialized to attack different plant species. Here, we provide de novo chromosome-level genome assemblies of a cotton specialist population (Hap1) and a cucurbit specialist population (Hap3). We achieved this by using a combination of third-generation sequencing platforms, namely Illumina and Hi-C sequencing technologies. We were able to anchor a total of 334.89 Mb (scaffold N50 of 89.13 Mb) and 359.95 Mb (scaffold N50 of 68.88 Mb) to four chromosomes for Hap1 and Hap3, respectively. Moreover, our results showed that the X-chromosome of Hap3 (113.01 Mb) was significantly longer than that of Hap1 (100.26 Mb), with a high level of sequence conservation between the aphid species. We also report variation in the number of protein-coding genes and repeat sequences between Hap1 and Hap3. In particular, olfactory and gustatory receptor genes underwent a high level of gene duplication and expansion events in A. gossypii, including between Hap1 and Hap3. Moreover, we identified two glutathione S-transferase genes which underwent single gene duplications in Hap3, and tandem duplication and inversion events affecting the cytochrome P450 monooxygenase between Hap1 and Hap3, all of which include the CYP3 family. Our results illustrate the variance in the genomic composition of two specialized A. gossypii populations and provide a helpful resource for the study of aphid population evolution, host adaption and insecticide resistance.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Áfidos / Cucurbitaceae Límite: Animals Idioma: En Revista: Mol Ecol Resour Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Áfidos / Cucurbitaceae Límite: Animals Idioma: En Revista: Mol Ecol Resour Año: 2022 Tipo del documento: Article País de afiliación: China