UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.
STAR Protoc
; 2(4): 100878, 2021 12 17.
Article
en En
| MEDLINE
| ID: mdl-34604812
ABSTRACT
Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Técnicas de Amplificación de Ácido Nucleico
/
Sistemas CRISPR-Cas
/
SARS-CoV-2
/
COVID-19
Tipo de estudio:
Diagnostic_studies
/
Health_economic_evaluation
/
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Perú