Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum.
BMC Vet Res
; 17(1): 335, 2021 Oct 22.
Article
en En
| MEDLINE
| ID: mdl-34686208
ABSTRACT
BACKGROUND:
N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity.RESULTS:
In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro.CONCLUSIONS:
Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schistosoma japonicum
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Acetiltransferasas
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Esquistosomiasis Japónica
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Regulación Enzimológica de la Expresión Génica
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Proteínas del Helminto
Límite:
Animals
Idioma:
En
Revista:
BMC Vet Res
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2021
Tipo del documento:
Article