The role of DDK and Treslin-MTBP in coordinating replication licensing and pre-initiation complex formation.
Open Biol
; 11(10): 210121, 2021 10.
Article
en En
| MEDLINE
| ID: mdl-34699733
ABSTRACT
Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein. Immunodepletion and add-back experiments show that Treslin-MTBP is rate limiting for replication initiation. It is recruited onto chromatin before S phase starts and recruitment continues during S phase. We show that DDK activity both increases and strengthens the interaction of Treslin-MTBP with licensed chromatin. We also show that DDK activity cooperates with CDK activity to drive the interaction of Treslin-MTBP with TopBP1 which is a regulated crucial step in pre-initiation complex formation. These results suggest how DDK works together with CDKs to regulate Treslin-MTBP and plays a crucial in selecting which origins will undergo initiation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Xenopus laevis
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Cromatina
/
Proteínas Portadoras
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Proteínas Serina-Treonina Quinasas
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Proteínas de Ciclo Celular
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Proteínas de Xenopus
Límite:
Animals
Idioma:
En
Revista:
Open Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido