[HPLC fingerprint of Tibetan medicinal plant â³Banggaâ³ and chemical components analysis of its two original plants by LC-MS/MS].
Zhongguo Zhong Yao Za Zhi
; 46(19): 5027-5037, 2021 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-34738398
ABSTRACT
To obtain the chemical profile of Tibetan medicinal plant â³Banggaâ³, the present study established the HPLC fingerprint of â³Banggaâ³ and inferred common chemical constituents of its two original plants, Aconitum tanguticum and A. naviculare by LC-MS. The HPLC analysis was performed on a Kromasil 100 C_8 column(4.6 mm×250 mm, 5 µm) with acetonitrile(A)-0.1% formic acid in water(B) as mobile phase in a gradient elution mode. Besides, the flow rate was set at 1 mL·min~(-1) and the column temperature was 35 â. The detection wavelength was set at 255 nm and the injection volume was 10 µL. Seventeen batches of â³Banggaâ³ samples were analyzed and the HPLC fingerprint was established under the above conditions. Similarity evaluation was performed using Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012). As a result, 16 common peaks were selec-ted and the similarity values of 17 batches of â³Banggaâ³ were in the range of 0.702-0.966. Furthermore, one batch of A. tanguticum and one batch of A. naviculare were analyzed by LC-MS/MS and 74 common compounds were inferred, including 10 phenolic acids, 26 flavonoids, and 38 alkaloids. The established method, with good separation and strong specificity, is simple and feasible, and can be used for the quality control of â³Banggaâ³ and identification of its two original plants. A. tanguticum and A. naviculare are similar in chemical composition and component content, but are quite different in the content of flavonoids.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plantas Medicinales
/
Medicamentos Herbarios Chinos
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2021
Tipo del documento:
Article
País de afiliación:
China