Your browser doesn't support javascript.
loading
[Expression of hepatocyte nuclear factor 4γ in gastric carcinoma and its role in cell proliferation and stemness].
Jia, Y X; Tang, H; Chang, Z W; Fan, H J; Guan, X Y; Qin, Y R.
Afiliación
  • Jia YX; Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
  • Tang H; Department of Oncology, Cancer Hospital Affiliated Zhengzhou University, Zhengzhou 450003, China.
  • Chang ZW; Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
  • Fan HJ; Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
  • Guan XY; Department of Clinical Oncology, the University of Hong Kong, Hong Kong 999077, China.
  • Qin YR; Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
Zhonghua Zhong Liu Za Zhi ; 43(11): 1164-1169, 2021 Nov 23.
Article en Zh | MEDLINE | ID: mdl-34794218
Objective: To explore the role and molecular mechanism of hepatocyte nuclear factor 4γ (HNF4γ) in proliferation and stemness of gastric cancer. Methods: A total of 102 cases of paraffin-embedded gastric cancer tissues and matched adjacent gastric tissues and 42 cases of fresh-frozen tissues derived from gastric patients who received radical gastrectomy were collected from the First Affiliated Hospital of Zhengzhou University between 2012 to 2015. The expression of HNF4γ was tested by immunohistochemical staining, quantitative real-time polymerase chain reaction (qRT-PCR). HNF4γ overexpressed (AGS-HNF4γ) and shRNA silenced (HGC27-shHNF4γ) gastric cell lines were established. The effects of HNF4γ on cell proliferation and stemness were verified by XTT, clone formation and sphere formation assay. The expression of CD44 was detected by western blot. Results: The mRNA expression level of HNF4γ in fresh-frozen gastric cancer tissue was (12.43±2.702), which was significantly higher than (3.639±1.109) in normal tissue (P<0.001). The high protein expression rate of HNF4γ in paraffin-embedded gastric cancer tissues was 41.2% (42/102), which was significantly higher than 8.8% (9/102) in normal gastric mucosa tissue (P< 0.001). The protein expression of HNF4γ was closely related to the tumor differentiation, infiltration depth, lymph node metastasis and tumor stage (P<0.05). The median survival interval of patients with HNF4γ high expression was 25 months, the 3-year survival rate was 4.8% (2/42), significantly lower than 38 months and 51.7% (31/60) of patients with normal HNF4γ expression (P<0.001). The proliferation and CD44 protein expression of AGS-HNF4γ cells were significantly higher than those of the AGS-Vector cells. The number of clone formation, sphere formation rate of AGS-HNF4γ cells were 243.5±24.5 and (83.5±3.9)%, significantly higher than 81.0±16.0 and (21.8±5.6)% of AGS-Vector cells (P=0.030 and P=0.010, respectively). The proliferation and CD44 protein expression of HGC27-shHNF4 cells were significantly lower than those of the HGC27-vector cells. The number of clone formation, sphere formation rate of HGC27-shHNF4 cells were 26.0±1.0 and (20.8±8.4)%, significantly higher than 83.5±4.5 and (72.5±4.8)% of HGC27-vector cells (P=0.006 and P=0.030, respectively). Conclusions: HNF4γ is upregulated in the gastric cancer tissues and related with the poor prognosis of patients with gastric cancer. Overexpression of HNF4γ promotes the proliferation and remains the stemness of gastric cancer cells by upregulating the expression of CD44.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Carcinoma / Factor Nuclear 4 del Hepatocito Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhonghua Zhong Liu Za Zhi Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Carcinoma / Factor Nuclear 4 del Hepatocito Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhonghua Zhong Liu Za Zhi Año: 2021 Tipo del documento: Article País de afiliación: China